Liquid chromatography-tandem mass spectrometry method for simultaneous determination of seven commonly used anticancer drugs in human plasma
Publication year: 2012Source:
Journal of Chromatography B
Jingya Zhou, Shouhong Gao, Feng Zhang, Bo Jiang, Qin Zhan, Fei Cai, Jingxian Li, Wansheng Chen This paper describes the development and validation of a novel, general liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of cyclophosphamide, ifosfamide, irinotecan, etoposide, gemcitabine, carboplatin and pemetrexed concentrations in human plasma. Samples were prepared by two kinds of extraction method and analyzed using a gradient separation over an Atlantis T3-C18 column (2.1×100mm, 3μm, Waters). Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (0.1% formic acid and 10mM ammonium acetate) at a flow rate of 0.25mL/min. Linear coefficients of correlation were >0.992 for all analytes. The intra- and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 9.2%, while the accuracy was within ±10.5%. The mean recovery of all the analytes ranged from 50.0 to 81.0%. This method was successfully applied to clinical samples from cancer patients.
Highlights ► We developed a novel, sensitive, reproducible, and accurate LC-MS/MS method. ► This method could simultaneous determination of seven anticancer drugs in human plasma. ► This new method was successfully applied to clinical samples from cancer patients.
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ScienceDirect Publication: Journal of Chromatography B.