A novel matrix additive, MCM-41-type mesoporous silica nanoparticles, used for analysis of peptides by MALDI-FT/ICRMS
Publication year: 2012Source:
Chen Jun, He Tianxi, Fan Xuemei Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was thought to be unsuitable for the analysis of low-molecular-weight species since matrix peak noise made it difficult to identify analyte ions. In this study, MCM-41-type mesoporous silica nanoparticles (MSNs) was used as a matrix additive with saturated 2,5-dihydroxybenzoic acid (DHB) or α-cyano-4-hydroxycinnannic acid (CHCA) solution for the analysis of five peptides of a wide range molecular weight, Leu-Val,Phe-Val,Leu-Phe, bradykinin fragment 1–7,angiotensin II and ACTH fragment18–39. It was showed that MSNs was capable of reducing matrix peaks with laser power lower than 34% of saturated DHB especially for low polarity analyte or CHCA especially for high polarity analyte with molecular weight less than 500 regardless of the analyte-to-matrix ratio. In addition, MSNs could suppress ionization of oligopeptide and polypeptide. The way MSNs exerted its effect was supposed to be via the attraction between surface silanol groups and analyte.
highlights ► With MSNs as matrix additive, saturated DHB aqueous solution could be used due to there was only need to confirm appropriate laser power but not analyte-to-matrix ratio to reduce matrix ions. ► DHB coupled with MSNs could be useful for the detection of molecule below 500Da with simple sample preparation.
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ScienceDirect Publication: Talanta.