Ultrahigh pressure extraction of lignan compounds from Dysosma versipellis and purification by high-speed counter-current chromatography
Publication year: 2012Source:
Journal of Chromatography B
Qing Zhu, Feng Liu, Meixia Xu, Xiaojing Lin, Xiao Wang Ultrahigh pressure extraction (UPE) was employed to extract podophyllotoxin and 4′-demethylpodophyllotoxin from Dysosma versipellis. The effects of extraction parameters including extraction solvents, pressure, time and solid/liquid ratio were investigated using a High Hydrostatic Pressure Processor. The optimal condition for UPE of the target compounds was 80% methanol, 200MPa of pressure, 1min of extraction time and 1:12 (g/mL) of solid/liquid ratio. Podophyllotoxin and 4′-demethylpodophyllotoxin in the crude extract were purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (10:10:8:12, v/v), and the fractions were analyzed by HPLC, ESI-MS and 1H-NMR. As a result, 73.7mg podophyllotoxin and 16.5mg 4′-demethylpodophyllotoxin with purities over 96% were obtained from 260mg crude sample in one-step separation.
Highlights ► To extract and purify lignans from Dysosna versipellis. ► Ultrahigh pressure extraction combined with HSCCC was applied. ► Two compounds with high purities were obtained with high yield. ► The method was very useful for the extraction and purification of podophyllotoxins
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ScienceDirect Publication: Journal of Chromatography B.