Development and validation of a high-throughput method for determination of nine fluoroquinolones residues in muscle of different animal species by liquid chromatography coupled to tandem mass spectrometry with low temperature clean up

Publication date: 27 October 2017
Source:Journal of Chromatography A, Volume 1521
Author(s): Fabiano Barreto, Cristina B.D. Ribeiro, Rodrigo Barcellos Hoff, Teresa Dalla Costa
This work describes the development and validation of a quantitative and confirmatory method to determination of nine fluoroquinolones residues in poultry, bovine, swine and fish muscle using LC–MS/MS. Sample preparation was based in a fast solvent extraction with acetonitrile with 1% of formic acid followed by a low-temperature clean up procedure and centrifugation, without further steps. The recoveries ranged between 79% and 115%. The concentration work range was 0–200μgkg−1. The LOD and LOQ were 5 and 10μgkg−1, respectively. This high-throughput method was reliable for identification and confirmation of nine relevant compounds in food-producing animal tissues. Validation procedure was performed according the Directive 2002/657/EC criteria and the method showed fitness to purpose in terms of precision and reproducibility as well as for the other performance parameters.

Publication date: 27 October 2017
Source:Journal of Chromatography A, Volume 1521

Author(s): Fabiano Barreto, Cristina B.D. Ribeiro, Rodrigo Barcellos Hoff, Teresa Dalla Costa

This work describes the development and validation of a quantitative and confirmatory method to determination of nine fluoroquinolones residues in poultry, bovine, swine and fish muscle using LC–MS/MS. Sample preparation was based in a fast solvent extraction with acetonitrile with 1% of formic acid followed by a low-temperature clean up procedure and centrifugation, without further steps. The recoveries ranged between 79% and 115%. The concentration work range was 0–200μgkg−1. The LOD and LOQ were 5 and 10μgkg−1, respectively. This high-throughput method was reliable for identification and confirmation of nine relevant compounds in food-producing animal tissues. Validation procedure was performed according the Directive 2002/657/EC criteria and the method showed fitness to purpose in terms of precision and reproducibility as well as for the other performance parameters.